English translation for "helper plasmid"

辅助质体

Related Translations:

helper:  n.1.帮手，助手。2.起救助作用的东西。

helpers:  帮助物体工具栏辅助对象辅助工助手；助理工人

home helper:  家务辅助器

helper classes:  助手类

tape helper:  卷尺辅助对象

rotary helper:  司钻助手

helper constituents:  辅助成分

living helper:  生活帮手

protractor helper:  量角器辅助对象

helper grade:  辅助坡度

Example Sentences:

	1.	4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction 构建宿主菌基因精确定位突变株31bk ( arod : : arobkan ~ r )为了改变代谢途径脱氢奎尼酸( dhq )分支点上的代谢流量，使之充分流向目的产物奎尼酸合成方向，利用基因打靶技术构建了31884宿主菌arod基因精确定位插入突变体，使dhq脱水酶( dhqase )失活，阻断了碳代谢流流向芳香氨基酸生成的方向，同时用同源重组的方法将arob基因定位整合入染色体上，解除了限速酶对碳代谢流通过共同途径到达dhq的阻遏影响，并减轻代谢负担。
	2.	The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l 同时将该目的基因插入到杆状病毒表达系统的供体质粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位点间，经酶切、 pcr鉴定后，将重组的供体质粒gp1 - fast转化到含有杆状病毒和辅助质粒的dh10b _ ( ac )感受态细胞中，获得了表达gpvh1株vp1的重组杆状病毒gp1 - bac 。
	3.	Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073 . marker exchange was achieved by selection on yma containing sm , km , gm , spc et al four kinds of antibiotics . mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively 随后，利用突变质粒pgxn217诱变慢生型大豆根瘤菌菌株gx201 ，获得了gx201的突变体菌株gx217 ；将pgxn201的3 . 4kbecori片段与plarf3连接获得亚克隆pgxn201cl ，然后将pgxn201cl导入突变体菌株gx217 ，构建了gx217的功能互补菌株rgx217 。

Similar Words：

"helper locomotive" English translation, "helper lymphocyte t" English translation, "helper motor" English translation, "helper object" English translation, "helper phage" English translation, "helper post" English translation, "helper roll" English translation, "helper spring" English translation, "helper stringer" English translation, "helper t cell" English translation